He is a video game character affiliated with the Sonic the Hedgehog series. He made his debut in Sonic the Hedgehog 3, becoming popular enough to earn top billing with Sega’s flagship star in Sonic & Knuckles. Sonic is the speedy type, while Knuckles is slower but stronger.
According to the website Know Your Meme, YouTube user GregZilla published a review in February 2017 for the game Sonic Lost World. It features a parody animation of Knuckles that looks shorter and fatter compared to his original form. The depiction of Knuckles was later turned into a 3D model by a user of the website DeviantArt.
Ugandan Knuckles is inspired partly by the film "Who Killed Captain Alex?", a low-budget Ugandan action/comedy released in 2010. Ugandan Knuckles’ catchphrase “do you know the way” spoken with an African accent was inspired by a similar line in the movie (it is also phonetically spelled "do you know de wey?")
Through an app called VRChat, a virtual reality social game. With a 3D model in place, users were able to populate VRChat with countless Ugandan Knuckles, all saying the same phrase and sometimes making clucking sounds, an apparent imitation of the sounds that are part of southern African languages.
Outlets including New York Magazine and gaming news site Kotaku note when users are mimicking African accents or clicking sounds, they will crack jokes about the Ebola virus. In one YouTube video, a version of Knuckles is shown wearing what appears to be blackface with the image of a watermelon across its chest.
If Knuckles' path away from innocence sounds familiar, it's because we've sort of seen this unfold before.